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Image Search Results
Journal: Laboratory investigation; a journal of technical methods and pathology
Article Title: Functional significance of erythropoietin receptor expression in breast cancer.
doi: 10.1097/01.lab.0000020415.72863.40
Figure Lengend Snippet: Figure 1. Results of immunohistochemical studies. Contiguous tissue sections from breast tumors were immunostained to detect EPO, EpoR, or presence of pimonidazole adducts as a marker for hypoxia. Panels A, B, and C, Immunostaining of contiguous sections from the same tumor showing reactivity for EpoR (A), EPO (B), and pimonidazole adducts (C). Panels D and E, Immunostaining of contiguous sections for EpoR (D) and EPO (E), demonstrating EpoR immunoreactivity and no EPO expression. Panel F, High magnification of tissue section showing strong EpoR immunoreactivity in breast cancer cells. Panel G, Z-chamber consists of plexiglass rings (1), with an access port on the side (2), and covered with 180 m nylon mesh on both sides allowing the in-growth of vessels (3). Panels H and I, Representative section of tumor removed from Z-chamber stained with hematoxylin and eosin (H & E). Panel H, Histology of typical tumor formation at Day 7 after implantation of the T-ZCs. The maximal tumor depth (green arrow) is representative of negative control chambers containing rat mammary adenocarcinoma R3230 Ac cells and vehicle. Panel I, Reduction in maximal tumor depth (green arrow) observed in a T-ZC containing recombinant soluble EpoR (compare panels H and I). A to F, bar indicates 100 m; H and I, bar indicates 500 m.
Article Snippet: In these experiments,
Techniques: Immunohistochemical staining, Marker, Immunostaining, Expressing, Staining, Negative Control, Recombinant
Journal: Laboratory investigation; a journal of technical methods and pathology
Article Title: Functional significance of erythropoietin receptor expression in breast cancer.
doi: 10.1097/01.lab.0000020415.72863.40
Figure Lengend Snippet: Figure 3. Inhibition of tumor growth by EPO-EpoR antagonists. Rat R3230Ac mammary adenocarcinoma cells were injected into tumor Z-chambers as described in “Materials and Methods.” The chambers contained one-time doses each of recombinant sEpoR, MAB-287 anti-EPO neutralizing antibody, AG-490 Jak2 tyrosine kinase inhibitor, or vehicle (Control). Treatment groups are indicated on top of the chart and final concentrations of each compound are indicated on the x axis. At Day 7, the tumors in the Z-chambers were removed and H&E staining was performed. Quantitative analysis of maximal tumor depth was carried out in each treatment group and is expressed as a percentage of control, as indicated in the y axis. The data is expressed as mean standard error of the mean. To determine significant effects of treatment, the mean of each treatment group was compared with control using the Students t test (two-tail) and p values for each comparison are indicated above each bar. * p 0.05. NS, not significant.
Article Snippet: In these experiments,
Techniques: Inhibition, Injection, Recombinant, Control, Staining, Comparison